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- Title
خصوصیات مولکولی، سرولوژیکی و بیولوژیکی دو فیتوپلاسمای متعلق به زیر گروه C درگروه آران ای ریبوزومی 16SrII همراه با بیماری جاروک یونجه در استان های یزد و فارس
- Authors
حسینی, سیدعلیرضا اسمعیل زاده; خداکرمیان, غلام; صالحی, محمد; برتاچینی, آسوآنتا
- Abstract
Alfalfa witches' broom (AWB) is a limiting factor for alfalfa growth and production in Iran, especially in the tropical regions of the country. AWB phytoplasma strains from two severely affected areas, Chahgeer (Abarkooh, Yazd province) and Juyom (Larestan, Fars province), were compared for main biologic, serologic and molecular characteristics. Based on disease symptoms in alfalfa farms, Chahgeer and Juyom AWB (CAWB and JAWB, respectively) strains were not differentiable. In dodder inoculated periwinkle and tomato plants JAWB phytoplasma induced stronger little leaf compared to the one induced by CAWB phytoplasma. In these experiments the two JAWB and CAWB phytoplasma strains are confirmed as vectored by different leafhopper species, Orosius albicinctus and Circulifer haematoceps respectively. Based on 16S rRNA gene and 16S-23S intergenic spacer region sequences, CAWB and JAWB were not differentiable, however no serologic relationship was observed between the two phytoplasmas in ELISA and DIBA tests using polyclonal antibodies prepared against each of them. Due to the lack of serological relationship, different insect vectors and induction of different symptoms in common host plants, CAWB and JAWB phytoplasmas should be considered as two different AWB strains both belonging to 16SrII-C subgroup.
- Publication
Iranian Journal of Plant Pathology, 2017, Vol 53, Issue 2, p165
- ISSN
0006-2774
- Publication type
Academic Journal