We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Enhanced oil accumulation in tobacco (Nicotiana tabacum L.) leaves by ectopic overexpression of VgDGAT1a for renewable production of biofuels.
- Authors
Chang-Yong Gao; Xue Mao; Hong-Qin Shang; Fang Li; Run-Zhi Li
- Abstract
To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5-5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of α-linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.
- Publication
Current Science (00113891), 2018, Vol 114, Issue 6, p1234
- ISSN
0011-3891
- Publication type
Academic Journal
- DOI
10.18520/cs/v114/i06/1234-1240