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- Title
Isolation and characterization of NBS-encoding disease resistance gene analogs in watermelon against fusarium wilt.
- Authors
Reddy, Anand C.; Lavanya, B.; Tejaswi, T.; Rao, E. Sreenivasa; Reddy, D. C. Lakshmana
- Abstract
Fusarium wilt (Fusarium oxysporum f. sp. niveum) in watermelon is one of the deadliest diseases around the globe, and availability of disease-resistant varieties is moderate. Disease management utilizing resistance genes (R-genes)/resistance gene analogs (RGAs) has proven to be a promising and successful approach. In the present study, six watermelon RGAs were isolated from wild, fusarium wilt resistant genotype IIHR-82 (Citrullus lanatus var. citroides) using degenerate primers that identify nucleotide binding site-leucine-rich repeat (NBS-LRR) regions. Multiple sequence alignment of these RGAs identified the characteristic NBS- LRR motif, and BLASTp search revealed similarity of these RGAs with other pathogenesis-related proteins. Phylogeny and motif analysis revealed genetic diversity of RGAs within those isolated from watermelon and with other plant R-genes. The watermelon RGAs isolated in this study contained both TIR-NBS-LRR (TNL) and non-TIR-NBS-LRR (CNL) classes of Rgenes. Protein secondary structure prediction of these watermelon RGAs revealed the composition of proteins, including α -helix, β -strand, disordered region and other template-related information. Watermelon RGAs identified in the present study will help in the development of RGA-based markers for resistance to fusarium wilt of watermelon.
- Publication
Current Science (00113891), 2019, Vol 117, Issue 4, p617
- ISSN
0011-3891
- Publication type
Academic Journal
- DOI
10.18520/cs/v117/i4/617-626