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- Title
Regulation of Myocardial Glucose Utilization by Intravascular Lipolysis of Triglycerides (IVTL) In Vivo in Rats.
- Authors
Ménard, Sébastien L.; Xiuli Ci; Lavoie, François; Frisch, Frédérique; Normand-lauzière, François; Rousseau, Jacques A.; Cadorette, Jules; Vanlier, Johan E.; Bénard, François; Bentourkia, M'hamed; Lecomte, Roger; Carpentier, Andre
- Abstract
In type 2 diabetes, almost all of energy requirements of the heart are fulfilled by fatty acids at the expense of reduced glucose utilization. Although increased availability of fatty acids from the plasma free fatty acid pool explains at least partly this observation, the contribution of IVTL to this phenomenon is unknown. In vivo inhibition of IVTL with injection of Triton WR-1339 (TRI) vs. saline (SAL) was performed during a euglycemic hyperinsulinemic clamp to standardize blood glucose levels in Wistar rats with and without an IV infusion of Intralipid 20% (IL+ vs. IL-) to increase circulating triglycerides. [sup 13]NH[sub 3], [sup 11]C-acetate and [sup 18]F-deoxyglucose kinetics by micro-positron emission tomography (microTEP) with multicompartmental models were used to determine myocardial blood flow, total oxidative metabolism, and myocardial metabolic rate of glucose (MMRG), respectively. As expected, IL+ reduced MMRG by 44% during SAL (P=0.06). This diminution was associated with a 63% reduction of coronary blood flow (P=0.006). TRI reduced myocardial incorporation of ³H-triolein but not [sup 14]C-bromopalmitate. TRI did not significantly change myocardial blood flow nor total oxidative metabolism. However, TRI reduced MMRG in IL- but tended to increase MMRG in IL+ (P=0.03 for reversal in trends). Our results suggest that regulation of MMRG by IVTL is complex and depends on exogenous lipid availability. More studies are needed to determine whether IVTL contributes to altered myocardial glucose utilization in type 2 diabetes.
- Publication
Diabetes, 2007, Vol 56, pA16
- ISSN
0012-1797
- Publication type
Academic Journal