We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Exhaled Volatile Organic Compounds During Hyperglycemia and Euglycemia/Hyperinsulinemia in Healthy Males.
- Authors
Galassetti, Pietro; Oliver, Stacy; Midyett, Jason; Flores, Rebecca; Pontello, Andria; Rosa, Jaime; Blake, Donald
- Abstract
Recent advances in analytical techniques allow reliable measurement in the human breath of very small concentrations of volatile organic compounds (VOCs), many of which may be by-products of human metabolism. VOCs are therefore potentially ideal non-invasive markers of endogenous biochemical processes. We have previously reported increased exhaled methyl nitrate in hyperglycemic diabetic children, but not in healthy controls, results explainable by the lack of a hyperinsulinemic response to hyperglycemia in the diabetic. To test this hypothesis we generated hyperinsulinemia with and without hyperglycemia, and measured numerous glucoregulatory plasma variables and exhaled gas concentrations. 10 healthy males (26.8±1.4 yrs) participated. After an overnight fast, i.v. catheters were placed in veins of both arms for blood draws and dextrose/insulin infusions. Procedures lasted 240 min, including baseline (t = 0-60 min), hyperglycemia (t = 60-150; plasma glucose ∼ 220 mg/dl via dextrose infusion + endogenous compensatory hyperinsulinemia); and euglycemia-hyperinsulinemia (t = 150-240 min; glucose infusion continued to clamp euglycemia; constant insulin infusion @ 1.5 mU/kg/min). Plasma for later metabolite assays, room air and exhaled gases, were sampled at 12 separate time-points. As shown in figure, changes in plasma insulin, independent of concomitant glucose levels, were paralleled by proportional but opposite changes in FFA (and Other lipid metabolism variables such as glycerol, ketones). The kinetic profile of exhaled methyl nitrate closely resembled that of circulating lipids and exhaled ketones (acetone). In 10 healthy young males, during hyperinsulinemia, independent of the presence of hyperglycemia, exhaled methyl nitrate decreased proportionally to the increase in plasma insulin, and to the decrease in plasma lipids and ketones. Our observations indicate the potential use of methyl nitrate as a non-invasive biomarker of systemic inflammation, as related to lipid levels, and of hypo-insulinemic hyperglycemia, typical of Type 1 diabetes.
- Publication
Diabetes, 2007, Vol 56, pA108
- ISSN
0012-1797
- Publication type
Academic Journal