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- Title
Upregulation of PTEN Gene Expression in Adipocytes by Oxidized Low Density Lipoprotein: Implication for Impairment of Insulin Signaling in Obesity.
- Authors
Ajuwon, Kolapo M.; Franklin, Dru M.; Mochida, Rumi
- Abstract
Obesity and metabolic syndrome are associated with elevated levels of circulating oxidized low density lipoproteins (oxLDL). There is positive association between circulating oxLDL and insulin. Since both obesity and metabolic syndrome are associated with significant impairment of insulin signaling, we wanted to test the hypothesis that oxLDL is a mediator of impaired insulin action. We examined the regulation of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a major negative regulator of insulin signaling in adipocyte by oxLDL. Fully differentiated 3T3-L1 adipocytes were treated with 10, 50 and 100 µg/mL copper oxidized LDL for 24 hours in serum free media. Whole cell lysate and mRNA were recovered for immunoblotting and real time PCR for PTEN. OxLDL significantly (P<0.05) induces the mRNA and protein expression of PTEN. Immunoblotting with a phospho-PTEN (Ser380/Thr382/383) antibody also shows increased phosphporylation of PTEN by oxLDL. While we are currently investigating the overall implication of PTEN induction in the context of insulin resistance, these results implicate oxLDL in the onset of insulin resistance in the adipocyte.
- Publication
Diabetes, 2007, Vol 56, pA364
- ISSN
0012-1797
- Publication type
Academic Journal