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- Title
Lipid Deposition in Peripheral Blood Mononuclear Cells (pbmc) - A Link Between Insulin Resistance, Post Prandial Hyperglycemia and Arteriosclerosis?
- Authors
Weiss, Ram; Horowitz, Miri; Behar, Vered; Carley, William; Ziv, Ehud; Hirshberg, Boaz
- Abstract
A tight relation has previously been demonstrated between insulin resistance and lipid deposition in liver and skeletal muscle as the "driving force" of metabolic syndrome. The mechanistic link between peripheral insulin resistance, post prandial hyperglycemia, and arteriosclerosis is not clear. We postulated that the dynamics of lipid deposition within circulating peripheral blood mononuclear cells (PBMC), known to migrate into many tissues, may represent a link between these factors. Diabetes prone Psammomys obesus were fed a high-energy diet (HED). Lipid accumulation within blood monocytes under fasting and non-fasting conditions was assessed during the development of insulin resistance and hyperglycemia using a high resolution imaging technology of wet samples in a scanning electron microscope (WETSEM™). Twenty cells per sample were analyzed and the results were expressed as the mean. Fasting and non-fasting levels of lipid, (e.g. total fat area per monocyte (PBMC)), were comparable (0.7 ± 0.05 vs. 0.63 ± 0.06 µm², P=0.78) in animals at baseline and on a low energy diet (LED). In diabetic animals on HED the area of lipid droplets was larger in the non-fasting state than in the fasting state (1.06 ± 0.21 vs. 0.45 ± 0.2 µm², p=0.06). Thus, in the non-fasting HED state, diabetic animals had twice as much fat area in comparison to non-diabetic animals. Hyperglycemic, insulin resistant Psammomys Obesus have greater lipid deposition in blood derived monocytes in the non-fasting state compared to their non-diabetic counterparts. Lipid deposition in monocytes may be a link between post prandial hyperglycemia, insulin resistance and arteriosclerosis.
- Publication
Diabetes, 2007, Vol 56, pA465
- ISSN
0012-1797
- Publication type
Academic Journal