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- Title
Glucose Administration Inhibits the Hepatic Activation of Gluconeogenesis Promoted by Insulin Induced-Hypoglycemia.
- Authors
Batista, Marcia R.; Galende, Sharize B.; Vardanega - Peicher, Marcia; Bazotte, Roberto B.
- Abstract
The activation of hepatic gluconeogenesis in male Wistar adult 6 h fasted rats during insulin-induced hypoglycemia was previously demonstrated. In this study, the effects of intraperitoneal glucose (100 mg/kg) on the activation of liver gluconeogenesis promoted by insulin-induced hypoglycemia were determined. Thus, 6 h fasted rats that received intraperitoneal regular insulin (1 U/kg) and 30 min later intraperitoneal administration of vehicle (Control group) or glucose (Experimental group) were compared. All experiments were executed 60 min after insulin injection. Glycemia of Control and Experimental groups (N=I 5.325 ± 0.18 mM vs 3.39 ± 0.15 mM, respectively) were not different (P > 0.05). To investigate gluconeogenesis, liver perfusion experiments were performed. The results demonstrated that except to glycerol (N=5.585 mmol/g ± 0.40 vs 8.43 mmol/g ± 0.18, to Control and Experimental groups respectively), livers from rats which received intraperitoneal glucose, i.e., Experimental group, showed lower (P < 0.05) gluconeogenesis from L-alanine, L-glutamine, L-lactate or L-alanine + L-glutamine + L-lactate + glycerol. Therefore, the absence of glucose recovery after the glucose administration was mediated by an inhibition of hepatic gluconeogenesis.
- Publication
Diabetes, 2007, Vol 56, pA676
- ISSN
0012-1797
- Publication type
Academic Journal