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- Title
WPŁYW WARUNKÓW EKSTRAKCJI ORAZ BIOAKUMULACJI METALI NA WYNIK OZNACZANIA SZCZAWIANÓW ROZPUSZCZALNYCH (SZR) METODĄ ENZYMATYCZNO-SPEKTROFOTOMETRYCZNĄ W WODNYCH NAPARACH WYBRANYCH ROŚLIN LECZNICZYCH I HERBAT ZIELONYCH.
- Authors
Sperkowska, Beata; Bazylak, Grzegorz
- Abstract
The content of soluble oxalates (SZR) was determined by using the enzymatic method (Trinity Biotech, Ireland) in the water infusions of seven popular dried medicinal plants with domestic origin (Saint John's Wort, chamomile, lime, fennel, peppermint, sage, nettle) and five dried green teas (China, Vietnam, Indonesia). The water infusion were prepared by three methods: 1) traditional extraction with boiling water of 100°C (EWW), 2) microwave radiation supported extraction at 80°C (EWPM) and 3) ultrasound supported extraction (EWU). The effect of increasing temperature (40 or 60°C) and extraction time (15 or 30 min) of the EWU process on the amount of SZR in obtained water infusions from studied plant materials were also determined. After the use of the EWW method the highest mean amount of SZR in the medicinal plant infusions 10,64 mg·dm-3 was observed. However, the highest mean content of SZR in the green teas 52,94 mg·dm-3 was observed after the use of EWPM method. This last amount was near five fold higher than mean amount of SZR observed in the medicinal plant infusion (9,34 mg·dm-3) prepared by EWPM method. The mean lowest amount of SZR was observed in studied infusions after its preparation by the EWU method in 15 min and at 40°C, i.e. 27,01 and 5,45 mg·dm-3, respectively, in the green teas and medicinal plants infusions. Irrespective of the EWU extraction time (15 or 30 min) the increasing temperature of this process from 40 to 60°C was leading to near 50% increase of the SZR content in obtained infusions of each kind of studied medicinal plant and green teas. However, the observed very sharp decrease in the SZR content in the green teas, Saint John's wort, chamomile and nettle infusions prepared by the EWU method in 15 min and at 40°C have been probably caused by the increasing amounts of the enzyme inhibiting and interfering species as manganese, iron or copper ions which leaking from the plant material in this extraction conditions. Irrespective of the used extraction method the observed SZR content was highly increased in the infusions prepared from leafs type materials (peppermint, sage, nettle) in comparison with the whole plant, inflorescence or fruit type materials (Saint John's wort, chamomile, lime, fennel). Beside of applied here extraction method and bioaccumulation intensity of Mn, Fe and Cu the increased grinding and bulk density of the leaf type green teas was accompanied by significantly enlarged content of SZR in the studied infusions.
- Publication
Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocawiu: Rolnictwo, 2016, Issue 621, p59
- ISSN
0137-1959
- Publication type
Academic Journal