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- Title
Comparison of Three Rapid Tests for the Detection of β-Lactamase Production among Bacterial Isolates.
- Authors
Jeong Woo Kang; Seonhwa Kim; Hae-Chul Park; Yangho Jang; Mamata Gurung; Jae-Young Song; Kwang-Jick Lee; Tae-Wan Kim
- Abstract
The current study compares the perfomiance and evaluated the practicality of three rapid detection methods for β-lactamase production among 48 veterinary bacterial isolates (16 S. aureus, 18 E. coli, and 14 S. typhimurium) in Korea. Antimicrobial susceptibility was determined by broth microdilution method and β-lactamase production was detected by iodometric, acidimetric, and chromogenic methods. Seventy five percent of S. aureus, 44% of E. coli, and 35% of S. typhimurium were resistant to penicillin, ampicillin, and amoxicillin, respectively. Overall, the iodometric test yielded the highest positive results (91.67%), followed by chromogenic test (66.67%) and acidimetric test (47.92%). The sensitivities and specificities of the three different tests varied for S. aureus, E. coli, and S. typhimurium isolates. Our results showed that these test methods may applicable to monitor β-lactamase production among various microorganisms and to predict β-lactam antibiotic resistance.
- Publication
Pakistan Veterinary Journal, 2016, Vol 36, Issue 3, p363
- ISSN
0253-8318
- Publication type
Academic Journal