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- Title
Regulation of adipogenesis by cytoskeleton remodelling is facilitated by acetyltransferase MEC-17-dependent acetylation of a-tubulin.
- Authors
Wulin YANG; Xiangxiang GUO; THEIN, Shermaine; Feng XU; SUGII, Shigeki; BAAS, Peter W.; RADDA, George K.; Weiping HAN
- Abstract
Cytoskeleton remodelling is a prerequisite step for the morphological transition from preadipocytes to mature adipocytes. Although microtubules play a pivotal role in organizing cellular structure, regulation of microtubule dynamics during adipogenesis remains unclear. In the present paper we show that acetylation of a-tubulin is up-regulated during adipogenesis, and adipocyte development is dependent on a-tubulin acetylation, as expression of an acetylation-resistant a-tubulin mutant significantly inhibits adipogenesis. Moreover, acetylation of a-tubulin is under the control of the acetyltransferase MEC- 17 and deacetylases SIRT2 (Sirtuin 2) and HDAC6 (histone deacetylase 6). Adipocyte development is inhibited in MEC-17- knockdown cells, but enhanced in MEC-17-overexpressing cells. Finally, we showthat katanin, a microtubule-severing proteinwith enhanced activity on acetylated a-tubulin, is actively involved in adipogenesis. We propose that co-ordinated up-regulation of a-tubulin acetylation initiates cytoskeleton remodelling by promoting a-tubulin severing by katanin which, in turn, allows expansion of lipid droplets and accelerates the morphological transition toward mature adipocytes.
- Publication
Biochemical Journal, 2013, Vol 449, Issue 3, p605
- ISSN
0264-6021
- Publication type
Academic Journal
- DOI
10.1042/BJ20121121