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- Title
Exploring the Effects of Mesenchymal Stem Cell and Cholecalciferol on Regeneration in Facial Nerve Damage.
- Authors
Nursaçan, Onur; Erkan, Mustafa; Şan, Furkan; Gönen, Zeynep Burçin; Yetkin, Mehmet Fatih; Yay, Arzu; Önder, Gözde Özge; Ünlü, Şaban Murat; Vural, Alperen; Sevim, Çağlar; Avcı, Deniz; Şahin, Mehmet ilhan
- Abstract
Objective: To evaluate the effects of adipose-derived mesenchymal stem cells (ADMSCs) and cholecalciferol (Vitamin D3, active form, calcitriol) on the regeneration of the facial nerve. Methods: 32 Sprague-Dawley rats were used. Right facial nerves of the rats were dissected and the main truncus of the nerve was pressed for 60 seconds with a sensor-fitted device to create a crush injury in each rat. The rats were evenly separated into four groups: Group 1 (positive control group): Physiological saline solution was applied to the damaged site, and the left facial nerves of these rats were evaluated as the sham group (negative control group). Group 2: Oral cholecalciferol (3500 IU/kg/week) was administered for four weeks. Group 3: 1x106 ADMSC was applied to the site of injury. Group 4: 1x106 ADMSC was applied to the site of injury in combination with oral cholecalciferol (3500 IU/kg/week) administered for four weeks. Nerve regeneration: Functional evaluation was performed by monitoring eye blink reflex at weekly follow-ups and with EMG (nerve transmission study) after four weeks; and histologic evaluation was made at the end of the fourth week by analyzing the number of axons and the diameter of myelinated axons in the nerve section. Results: In functional evaluation Group 3 and Group 4 were observed to show the fastest improvement (p<0.05). In EMG, compound muscle action potential (CMAP) amplitude was found significantly higher and CMAP latency was found significantly shorter in all experiment groups compared to the control group (p<0.05). In EMG, Groups 3 and 4 showed the most favorable results in terms of regeneration. In histologic evaluation, the most favorable axon morphometry and myelinization results were found in Group 4 (p<0.05). Groups 2 and 3 showed better results, but no statistically significant difference compared to the control group (p>0.05). Conclusion: In experimental facial nerve crush injury, ADMSC application combined with cholecalciferol treatment will functionally, electrophys-iologically and histologically increase nerve regeneration and remyelination.
- Publication
Turkish Archives of Otolaryngology / Türk Otolarengoloji Arsivi, 2019, Vol 57, pS12
- ISSN
0304-4793
- Publication type
Academic Journal
- DOI
10.5152/tao.2019.20012