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- Title
Genetic Profiling of CYP19 Gene Haplotype in Holstein Bulls.
- Authors
Habib, Hassan Nima; Al-Rishdy, Khalaf A. H.; AL-Hellou, Murthda F.
- Abstract
The CYP19 gene is a protein-coding gene that stimulates the transmutation of androgens to estrogens, aromatase activity is related to haplotypes of the CYP19 gene, therefore this study aimed to detect haplotypes of the CYP19 gene in the Holstein bulls. This study, conducted from October 2018 to June 2019. Forty-two Bulls, aged between 4-6 years old were used, which belonged to local farmers in Basra city, south of Iraq. The DNA was extracted from semen, PCR amplification was carried out to acquire the DNA sequence. The sequences were analyzed by using some Bioinformatics tools. The results showed four haplotypes in the CYP19 gene as a result of silent and missense mutations in comparison with the same gene in GenBank, all the haplotypes are recorded in GenBank, DDBJ and EMBL. These findings call for further studies to reveal the relationship of CYP19 gene haplotypes and semen characteristics to uncover bulls’ fertility.
- Publication
Indian Journal of Forensic Medicine & Toxicology, 2021, Vol 15, Issue 2, p2473
- ISSN
0973-9122
- Publication type
Academic Journal
- DOI
10.37506/ijfmt.v15i2.14743