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- Title
Detection of Campylobacter jejuni and Campylobacter coli by a PCR assay in Patients with Diarrhea.
- Authors
Abd, Rawa Abdalkreem; Mezal, Ezat Hussain
- Abstract
Campylobacter sp. is complex taxonomy, fastidious growth criteria, and biochemical inertness, identification of campylobacter in clinical samples by traditional cultures is problematic and slow. Because of that a rapid, responsive, and precise polymerase chain reaction (PCR) assay capable of detecting and differentiating Campylobacter jejuni (hipO) and Campylobacter coli (glyA) directly from human feces. Diagnostic PCR on template DNA extracted directly from the stool offers catchy advantages including the reduced time of analysis and detection of non-viable and non-cultivable bacteria contained in the sample. From a human patient with diarrhea by using conventional PCR assay. One hundred fecal samples were collected from patients suffering from watery, bloody, and mucous diarrhea, aged (1 month-60 years old) from both sexes (55Male, 45 Female). From many places in Thi-Qar province, Bint Alhuda Hospital, and Outpatient Clinics during the period from August 2020 to November 2020. Four isolates (4%) were diagnosed as genus C.coli and one isolate (1%) was diagnosed as genus C.jejuniby using glyA and hipO gene for C.coli and C.jejuni respectively.
- Publication
Indian Journal of Forensic Medicine & Toxicology, 2021, Vol 15, Issue 3, p3940
- ISSN
0973-9122
- Publication type
Academic Journal
- DOI
10.37506/ijfmt.v15i3.15912