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- Title
Pectic Oligosaccharides Derived from Chickpea (Cicer arietinum L.) Husk Pectin and Elucidation of their Role in Prebiotic and Antioxidant Activities.
- Authors
Shakuntala, S.; Mol, Praseeda; Muralikrishna, G.
- Abstract
Chickpea husk rich in non-starch polysaccharides (NSP) were extracted sequentially using cold water, hot water, 0.5% ammonium oxalate (AO) and 0.5% EDTA in 0.9,0.4,4.4 and 3.8%) yields, respectively. Sugar composition of both AO and EDTA extract revealed the preponderance of galactose, xylose, arabinose and smaller amounts of rhamnose and mannose. Ammonium oxalate and EDTA extracts consisted of 71.2 and 62.4% uronic acid respectively, which is comparatively higher than that present in other dietary fibre components isolated from chickpea husk in the present study. Ammonium oxalate and EDTA extracted fractions which are rich in pectins were acid hydrolyzed to obtain pectic oligosaccharides. These oligosaccharides were detected by TLC and analysed by LC-MS. The antioxidant activity of pectic oligosaccharides derived from ammonium oxalate showed significant DPP1I radical scavenging activity compared to the EDTA derived pectic oligosaccharides and can be explored as novel antioxidant. The prebiotic properties of both AO and EDTA derived pectic oligosaccharides were studied using Lactobacillus acidophilus (NDRI Oil) and Bifidobacterium adolescentis (NDRI 236). Pectic oligosaccharides derived from the pectin isolated by ED TA extraction had relatively higher prebiotic activity than that derived from AO extracted pectin.
- Publication
Trends in Carbohydrate Research, 2014, Vol 6, Issue 4, p29
- ISSN
0975-0304
- Publication type
Academic Journal