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- Title
Characteristics and antioxidant activities of anthocyanin fraction in red dragon fruit peels (Hylocereus polyrhizus) extract.
- Authors
Prabowo, Imam; Utomo, Edi Priyo; Nurfaizy, Agung; Widodo, Aris; Widjajanto, Edi; Rahadju, Puji
- Abstract
Background: Dragon fruit has been reported for its high antiradical activities with the presence of phenolic compounds. Some significant characteristics of plant pigment are betacyanin, anthocyanins, and other flavonoids. This study aimed to determine the anthocyanin components, antioxidant activity, and Total phenolic content (TPC) of anthocyanin from the extract of red dragon fruit peels. Materials and Methods: The research was used methanol extraction, followed by other analyses, and thin-layer chromatography (TLC) to obtain the fractionated components. Anthocyanins were analyzed with a liquid chromatography-mass spectrometry (LC-MS) instrument to obtain cyanidin, malvidin, and delphinidin. Total phenolic content of anthocyanin was measured with Folin-Ciocaltau. Results: Antioxidant activity revealed 56.68% (crude extract), 36.91% (F3), and 36.24% (F9). This study used TLC followed by column chromatography for better separation of phenolics. Among the three samples, the highest radical scavenging activity demonstrated by lethal concentration50 was the crude extract, followed by F3 and F9. The LC-MS assay identified the single types of phenolic compounds such as cyaniding (31.63%), malvidin (7.37%), and delphinidin (7.53%). Conclusions: Red dragon fruit peel extract (Hylocereus polyrhizus) containing phytochemical compounds is an effective antioxidant from natural plant sources, with anticarcinogenic and anti-inflammation properties, and may help with other degenerative disorders.
- Publication
Drug Invention Today, 2019, Vol 12, Issue 4, p670
- ISSN
0975-7619
- Publication type
Academic Journal