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- Title
Comparison of the effect of Triphala, green tea, and 2% chlorhexidine on Streptococcus mutans biofilm formation.
- Authors
Haripriya, R.; Geetha, R. V.
- Abstract
Aim: This study aims to compare the antimicrobial activity of Triphala, green tea, and 2% chlorhexidine (CHX) on Streptococcus mutans biofilm on microtiter plate. Introduction: S. mutans is one of the major etiological factors of human dental caries. Triphala, a polyherbal preparation, which consists of equal amounts of fruits of three plants, namely, Amalaki Phyllanthus emblica, Haritaki, and Bahera in a fine powder form is said to have antimicrobial activity against oral pathogens. Green tea is said to have antibacterial properties against S. mutans and CHX is also known for its antimicrobial property against S. mutans. The antimicrobial efficacy of all the three substance is compared in this study using a microtiter plate. Materials and Methods: S. mutans was isolated and maintained in Tryptose soya agar at 4°C. Individual wells of sterile polystyrene 96-well flat bottom microtiter plates were filled with 200 ml of culture suspension of test organism. Different concentrations of Triphala, green tea, and CHX were added and incubated. After the incubation, the adherence of bacteria was fixed and stained by crystal violet. It was removed by acetone and readings were taken. Experiment was carried out in triplicate and their mean was taken for analysis. Results: CHX which is well known for its antimicrobial property had a greater efficacy in lower concentrations when compared with other two substances. In higher concentrations, Triphala was found to have greater efficacy than the other two substances.
- Publication
Drug Invention Today, 2019, Vol 12, Issue 9, p2034
- ISSN
0975-7619
- Publication type
Academic Journal