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- Title
Cytotoxic action of rutin isolated from Morinda citrifolia against hepatic carcinoma cell lines.
- Authors
Labh, Ankita Komal; Priya, V. Vishnu; Gayathri, R.
- Abstract
Aim: The goal of this study is to determine, quantitatively the cytotoxic action of the bioactive component, rutin isolated from Morinda citrifolia plant against hepatic carcinoma (HepG2) cell lines. Introduction: Hepatic cancer is one of the most common cancers in the world and has a high mortality rate. Alternative medicine for the treatment of cancer has been long under research. Rutin which is a flavonoid extracted from M. citrifolia (commonly known as Noni) has a variety of beneficial properties such as antiallergic, anti-inflammatory, antiproliferative, and anticarcinogenic properties. In this study, rutin has been used as a potential anticancer compound and its effects have been studied on HepG2 cell lines. Materials and Methods: HepG2 cell lines were procured from the National Centre for Cell Sciences, Pune, India. Ethanolic extract of M. citrifolia was prepared and rutin was extracted. 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was carried out. Siliphos was used as control. Absorbance was read on an enzymelinked immunosorbent assay reader at 570 nm. Cell viability percentage was calculated. Data were collected and results were represented using proper statistical methods. P value was calculated to check the statistical significance of the data collected. Results: The cell viability was found to decrease with an increase in the concentration of rutin used, P < 0.05. HepG2 cells treated with 100 µg/ml of Siliphos showed 25.1% cell viability and those treated with 30 µM/ml of rutin showed 30% cell viability. Cell viability of untreated HepG2 cells was 100%. Conclusion: Rutin has a highly significant cytotoxic action on HepG2 cell lines. Thus, we can conclude by saying that in the future rutin can be used as an alternative for conventional anticancer drugs.
- Publication
Drug Invention Today, 2019, Vol 12, Issue 9, p1904
- ISSN
0975-7619
- Publication type
Academic Journal