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- Title
Comparison of biofilm gene (gtf-B) and antibiotics resistance of Streptococcus mutans from dental caries in diabetic and non-diabetic patients.
- Authors
Al-Mazini, Mohammed A.
- Abstract
Background: Dental caries is a widespread disease even in developed countries in the human of oral cavity. The dental biofilm consists of various bacteria and some strains of Streptococcus mutans (S. mutans) have specific virulence factors for compete with another strains, this study investigates the relationship between diabetic and non-diabetic patients to determine three aims: In the first aim, to isolate and diagnosis of S. mutans from dental caries infection; in the second aim, for detection some of virulence factors; and in the third aim, the relationship between biofilm formation of S. mutans with gtf-B gene and antibiotics resistance against S. mutans isolates. Materials and Methods: The samples were identified depending on the biochemical test system of analytical profile index (API) Strep and Vitek system. Also, all isolates of S. mutans were determined for the virulence factors; biofilm formation using tissue culture plate method; determination of the gtf-B biofilm gene using Polymerase Chain Reaction and antibiotic sensitivity test. Results: A total of 100 bacterial isolates were identified from dental caries, which represent 50 diabetics and 50 non-diabetic patients for different ages. They were admitted to the dental clinics in Basra city. Bacterial isolates were identified according to biochemical tests, API 20 Strep and Vitek system. S. mutans have been proposed as the main etiological agent of dental caries in diabetic patients comparing with non-diabetic patients. S. mutans was highest percentage (40%), followed by Streptococcus sobrinus (20%), Staphylococcus epidermidis (14%), and Lactobacillus spp. (10%). Moreover, S. mutans from diabetic patients was significant difference in the age group of 50–60 years from non-diabetic patients. In the second aim (for detection some of virulence factors): The results showed that S. mutans isolates of dental caries of diabetic patients were significant differences (P ˂ 0.05) from non-diabetic patients according to detect of virulence factors. These virulence factors were included 85% of hemolysin, 90% of bacteriocin production, 95% of glucan production, and 100% of biofilm formation. Moreover, these virulence factors were very important factors for association with pathogenicity of S. mutans. In the third aim (the relationship between biofilm formation of S. mutans with gtf-B gene and antibiotics resistance against S. mutans isolates): Molecular study by PCR technique was used for the detection gtf-B gene, this gene was found as predominance in 20 (100%) of S. mutans in diabetic at significant differences (P ˂ 0.05) comparing with non-diabetic patients. In addition, this gtf-B gene produced glucosyltransferase which was responsible for the pathogenicity of S. mutans by the formation of biofilm and resistance of S. mutans trend five antibiotics (amoxicillin, chloramphenicol, erythromycin, tetracycline, and gentamycin). Conclusion: We can conclude from our study that S. mutans isolates from dental caries of diabetic patients were resistance for most antibiotics. The main reason of resistance was related with biofilm producing and producing of glucosyltransferase enzyme with express of gtf-B gene in these isolates.
- Publication
Drug Invention Today, 2020, Vol 13, Issue 5, p802
- ISSN
0975-7619
- Publication type
Academic Journal