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- Title
STABILITY INDICATING METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DAPAGLIFLOZIN AND SAXAGLIPTIN IN SYNTHETIC MIXTURE BY HPLC.
- Authors
Suthar, Nima; Marvaniya, Vanita; Patni, Pragnesh
- Abstract
A simple, rapid, economical, precise and accurate stability indicting HPLC method for simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form has been developed. A stability indicting high performance liquid chromatographic method was developed for the simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form has been developed. The separation was achieved by LC- 20 AT C18 (250mm x 4.6 mm x 2.6 µm) column and Buffer (pH 6.0): Acetonitrile (70:30) at a flow rate of 1 ml/min. Detection was carried out at 275 nm. Retention time 3.593 min and 5.263 for Dapagliflozin and Saxagliptin respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Dapagliflozin 10-30 µg/ml and for Saxagliptin 5-15 µg/ml. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Dapagliflozin and Saxagliptin in their combined dosage form. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial Combined dosage form.
- Publication
Pharma Science Monitor, 2018, Vol 9, Issue 2, p112
- ISSN
0976-9242
- Publication type
Academic Journal