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- Title
DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMELTANEOUS ESTIMATION OF CEFUROXIME SODIUM AND POTASSIUM CLAVULANATE IN BULK AND COMBINED DOSAGE FORM.
- Authors
Modi, J. D.; Patel, P. B.; Patel, Z. N.; Parikh, N. N.; Chaudhari, F. U.; Pradhan, P. K.; Upadhyay, U. M.
- Abstract
A Stability indicating RP-HPLC was developed and validated for determination of Cefuroxime Sodium (CEF) and Potassium Clavulanate (PCA). The RP-HPLC has shown adequate separation for Cefuroxime Sodium and Potassium Clavulanate from its degradation products. The separation was achieved on Hypersil BDS C18 column (250x4.6 mm i.d., 5 particle size) using a mobile phase composition of Acetonitrile: Phosphate buffer Ph4.5 (75: 25) with a flow rate of 1ml/min. Injection volume 20μl and wavelength of detection was kept at 275 nm the retention time of Cefuroxime sodium and Potassium Clavulanate were 3.06 and 7.63min respectively. Linearity was observed over concentration range of 10-40 μg/ ml for Cefuroxime Sodium and 6-20 μg/ml for Potassium Clavulanate. The mean recovery was found to be 100.08±0.68% and 99.95±0.67% for Cefuroxime Sodium and Potassium Clavulanate respectively The limit of detection was 0.34μg/ml and the limit of quantification was 0.112μg/ml for Cefuroxime Sodium and the limit of detection was 0.097μg/ml and the limit of quantification was 0.292μg/ml for Potassium Clavulanate. Cefuroxime Sodium and Potassium Clavulanate does not undergo any degradation so it can be said that Cefuroxime sodium and Potassium Clavulanate is stable to forced degradation conditions. This Method is simple, versatile, and reliable hence it can be used as stability indicating analytical Method for determination of Cefuroxime Sodium and Potassium Clavulanate in bulk and Marketed formulation.
- Publication
Pharma Science Monitor, 2014, Vol 5, Issue 2, p117
- ISSN
0976-9242
- Publication type
Academic Journal