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- Title
ISOLATION AND SCREENING OF SOIL MICROBES FOR EXTRACELLULAR CHITINASE ACTIVITY.
- Authors
A., Shivalee; Divatar, Mahesh; G., Sandhya; Ahmed, Sarfaraz; Lingappa, K.
- Abstract
Present investigation was aimed to isolate and screen microbiota from various soil samples collected from different regions of Kalaburagi, Karnataka, India for chitinase production. In simultaneous isolation and screening of microbes from fifty four soil samples, a total of seventy five microbial isolates were found with chitinolytic activity. These 75 isolates include 51 bacteria, 11 fungi and 13 actinomycetes. Among these, nineteen isolates exhibited sound, thirty three isolates exhibited moderate and twenty three isolates shown poor chitinase activity on chitin agar plate. Further, two bacterial, two actinomycetes and a fungal isolate with good chitinase activity were selected and subjected for chitinase production under submerged fermentation. As a result, comparatively an actinomycete KLSL-55 emerged as the highest enzyme producer with the activity of 84.66 IU.
- Publication
Journal of Advanced Scientific Research, 2016, Vol 7, Issue 2, p10
- ISSN
0976-9595
- Publication type
Academic Journal