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- Title
Effect of microRNA-17 on osteogenic differentiation of advanced glycation end products-stimulated human periodontal ligament stem cells.
- Authors
Deng Chao; Wu Yan; Yang Kun; Cui Xiaoxia; Liu Qi; Jin Yan
- Abstract
Objective This study aims to detect microRNA-17(mir-17) expression on the osteogenic differentiation of advanced glycation end products (AGEs)-stimulated hunman periodontal ligament stem cells (HPDLSCs) and to analyze the influence of these cells on this process. Methods HPDLSCs were isolated using limited dilution technique. After osteogenic differentiation occurred, different time points of mir-17 expression in the experimental groups were detected by real time polymerase chain reaction (PCR). The mir-17 overexpression and inhibition were evaluated using cell transfection technique. Differences in gene expressions were detected by real time PCR; differences in protein expressions were analyzed by Western blot. Results The mir-17 expression was reduced after osteogenic differentiation occurred at 3, 7, and 14 d compared with that in the control group (P<0.05). The expression levels of bone sialoprotein(BSP), Runt-related transcription factor-2 (Runx-2) and alkaline phosphatase (ALP) in the experimental groups were lower than those in the mimic control group when mir-17 expression increased. In addition, the protein expression levels of Runx-2 in the experimental groups were lower than those in the control group. The expression levels of BSP, Runx-2 and ALP in the experimental groups were higher than those in the inhibitor control group when mir-17 expression decreased. Likewise, the protein expression levels of Runx-2 in the experimental groups were higher than those in the control group. Conclusion AGEs inhibit the osteogenic differentiation of HPDLSCs by affecting mir-17 expression.
- Publication
West China Journal of Stomatology, 2015, Vol 33, Issue 1, p21
- ISSN
1000-1182
- Publication type
Academic Journal
- DOI
10.7518/hxkq.2015.01.005