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- Title
Isolation and identification of Streptococcus mutans strains with different genotype from clinical samples.
- Authors
Wang Chenglong; Su Donghua; Liu Jiaojiao; Chu Bingfeng; Li Shaohua; Xia Wei; Luo Yanpin; Yang Jiyone; Ding Hongmei; Zhao Qiang; Deng Bin; Xi Qing; Xu Juan; Shao Ningsheng
- Abstract
Objective To identify Streptococcus mutans (S. mutans) strains from clinical samples. Methods Plaque samples from caries-active and caries-free sites on enamel surfaces were obtained and cultivated for S. mutans isolation. Morphology, biochemistry, automatic microorganism analysis system and polymerase chain reaction using primers homologous to surface protein antigen I / II (spaP), glucosyltransferase B (gtfB) and dextranase (dexA) were used to identify S. mutans. Genotype of isolated S. mutans was determined by arbitrarily primed polymerase chain reaction. Results Forty-six strains of S. mutans were obtained from the 32 subjects and were identified as S. mutans by biochemistry, automatic microorganism analysis system and polymerase chain reaction. Five identical genotypes were found by arbitrarily primed polymerase chain reaction. Conclusion Forty-one strains of S. mutans with different genotype were obtained from clinical samples.
- Publication
West China Journal of Stomatology, 2013, Vol 31, Issue 1, p80
- ISSN
1000-1182
- Publication type
Academic Journal
- DOI
10.7518/hxkq.2013.01.020