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- Title
Effect of glimepiride on the glucose uptake of rat mandibular osteoblasts in hyperglycemia.
- Authors
Ma Pan; Tan Baosheng; Liu Hongchen; Ma Junlr; Gu Bin
- Abstract
Objective To explore the effect of glimepiride on the glucose uptake as well as glucose transporter (GLUT)-1 and GLUT-3 expression levels of rat mandibular osteoblasts in hyperglycemia. Methods Primary osteoblasts were isolated and cultured. Then, the cells were placed in an osteogenic medium containing two glucose concentrations (5.5 and 16.5 mmol-L-1), with or without glimepiride (10 μmol-L-1). Glucose uptake was determined by employing 18F-deoxyglucose(18F-FDG) in the cells, and GLUT-1 and GLUT-3 expression levels were evaluated by Western blot analysis. Results Glucose at 16.5 mmol-L-1significantly inhibited lsF-FDG uptake and downregulated GLUT-3 protein expression in osteoblasts. Hyperglycemia increased GLUT-1 protein expression. Glimepiride significantly increased glucose uptake and upregulated GLUT-1 and GLUT-3. Conclusion Glimepiride enhance the glucose transporter in rat osteoblasts at two different glucose concentrations.
- Publication
West China Journal of Stomatology, 2014, Vol 32, Issue 2, p125
- ISSN
1000-1182
- Publication type
Academic Journal
- DOI
10.7518/hxkq.2014.02.005