A new acylated C-glycosylflavone (1) was isolated from Trollius ledebouri Reichb together with nine known C-glycosyflavones (2-10). The structures were elucidated by spectroscopic methods, including HRMS, IR, ¹H and 13C NMR and 2D experiments (COSY, HMQC and HMBC). The anti-inflammatory activities of C-glycosyflavones were tested on TPA-induced mice ear edema (in vivo).