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- Title
Preparation of Conjugated Linoleic Acid from Aspergillus Oil.
- Authors
MENG Xian-hua; YANG Dong-liang; HU Jia-jia; DAI Yan-quan
- Abstract
An oleaginous filamentous fungi E1.3 belongs to the genus Aspergillus was isolated from the cambium in the woody stems of T. yunnanensis. After being cultured in shaking flasks for 7 days, total fungal oil was extracted from the dry biomass with petroleum ether by Soxhlet extraction. Gas chromatography/mass spectrometry was used to study the fatty acid profiles of the fungal oil. Consequently, the relative content of LA up to 31.6% is highest according to the normalization method of area. Then conjugated linoleic acids were prepared from fungal oil by alkali isomerization using KOH as catalyst and 2-butoxy ethanol as solvent for the first time. UV spectra was used to analyze the effects of four factors on the rate of conversion of LA, and the suitable conditions for the preparation process were found : reaction temperature of 140 °C, reaction time of 3 h, m (KOH) : m (oil) = 0.2: 1, and m (oil) : m (solvent) = 1 : 3. Under the optimum conditions, the average rate of conversion of LA could reach 86%. The results showed that fungal oil maybe a potential alternative source of vegetable oil as the raw materials of CLAs.
- Publication
Natural Product Research & Development, 2009, Vol 21, Issue 3, p492
- ISSN
1001-6880
- Publication type
Academic Journal