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- Title
不同组织来源间充质干细胞体外成骨分化能力的比较研究.
- Authors
赵刚; 刘微微; 高伟玮; 马洁
- Abstract
Objective To find the seed cells for the treatment of bone defects by comparing the osteogenesis capacity among adipose-derived stem cells (ASCs) ,human umbilical cord mesenchymal stem cells (UC-MSCs) ,and BMSCs. Methods The three MSCs were cultured in DMEM /Ham'sF-12 medium containing 10% fetal bovine serum. Using the third passage cells,cell growth curve was drawn using CCK8 method,and the cell surface antigens were measured using flow cytometry. Alkaline phosphatase (ALP) staining and Alizarin red staining were performed. The mineralized calcium nodules were quantitatively analyzed. The bone formation related genes were analyzed with RT-qPCR. Results The three MSCs were in the exponential growth stage at 3-5 days following incubation. Flow cytometry analysis showed that more than 97% cells were positive for CD44, CD90,and CD105. The negative rates of CD14,CD34,and CD45 were below 1%. ALP and Alizarin red staining displayed that three MSCs presented good mineralization ability by osteogenesis induced medium. RT-qPCR showed that the expression levels of osteogenic markers including Osterix,ALP,COLI,and OCN in three MSCs groups were higher than those in control group. UCMSCs experiment group expressed higher COLI gene than BMSCs in 9 days. ASCs expressed higher Osterix gene than BMSCs in 18 days. Conclusion ASCs and UC-MSCs have good osteogenic ability,which can be expected to be bone tissue engineering seed cells for the treatment of bone defects.
- Publication
Chinese Journal of Osteoporosis, 2017, Vol 23, Issue 5, p561
- ISSN
1006-7108
- Publication type
Academic Journal
- DOI
10.3969/j.issn.1006-7108.2017.05.001