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- Title
小规模多中心骨质疏松生物样本库的构建及应用探索 研究.
- Authors
李英华; 鹿彤; 施晓军; 陈飞; 胡晓晖; 崔云卿; 杨恭; 洪洋
- Abstract
Objective We aimed to construct a multicenter osteoporosis bio-bank and to provide data and bio-samples for perspective epidemiological surveys,personalized clinical therapy and public health preventive treatment of osteoporosis. Methods Ethical review based on the project of constructing osteoporosis sample bank. Using the data platform of community and hospital physical examination,subjects aged between 40 and 70 years without special diseases that affect bone and calcium metabolism and can corporate with long term follow-up visits were selected. Bone mineral density was measured at lumbar spine ( L1-4) ,femoral neck,wards region and total hip,using dual energy X-ray absorptiometry. Fasting urine samples ( 10 mL) ,and procoagulant and anticoagulant of peripheral blood ( 5 mL each) samples of all participants were collected according to the sample collecting rules of ISBER. DNA was extracted from the white cell of anticoagulant of peripheral blood and some susceptibility gene loci were genotyped. Urinary and blood index were measured once a year. Bone mineral density of those with T-score < - 2. 0 were measured every year,with T-score between - 1. 0 and - 2. 0 were measured every two years,and with T-score > - 1. 0 were measured every five years. The data of first measurement were regarded as baseline,and follow-up period is ten years. Results The osteoporosis bio-bank including 5000 subjects with data of BMD,blood samples,epidemiological data and some genetic information of osteoporosis candidate gene have been constructed. Conclusion Osteoporosis bio-bank is suitable for prospective or intervention studies of osteoporosis in Chinese geriatric cohort. It was the first time that sleep quality and sleep genetic information have been collected in the osteoporosis bio-bank.
- Publication
Chinese Journal of Osteoporosis, 2018, Vol 23, Issue 2, p148
- ISSN
1006-7108
- Publication type
Academic Journal
- DOI
10.3969/j.issn.1006-7108.2018.02.002