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- Title
FSHβ 在BMP9 诱导间充质干细胞成骨分化中的作用 研究.
- Authors
成亚琳; 曾继涛; 黄敏; 刘俊银; 涂小林; 刘宏
- Abstract
Objective To investigate the role of FSHβ (follicle-stimulating hormone β-subunit) in BMP9 (bone morphogenetic protein 9)-induced osteogenic differentiation of mesenchymal stem cells. Methods The cytochemical staining method was used to detect the change of ALP (alkaline phosphatase) expression in the cells on the 5th day after the treatment factors (recombinant adenovirus or γ-secretase inhibitor DAPT) were applied to MSCs. Quantitative real-time PCR (Q-PCR) was used to detect the changes of mRNA expression levels of BMP9, FSHβ and Notch signaling pathway target genes (Heyl). Alizarin Red S staining was used to detect the deposition of calcium salts in the cells on the 21st day after adenovirus-infection of MSCs. Results The ALP staining of FSHβ treatment group was not significantly changed compared with the GFP group; the ALP staining of BMP9 treatment group was significantly higher than the GFP group; and the ALP activity of BMP9 and FSHβ treatment group was significantly higher than the BMP9 treatment group. In addition, the expression levels of late osteogenic markers (calcium salt nodules) and Notch signaling target gene Heyl mRNA in the BMP9 treatment group were significantly higher than those in the GFP group (P<0.01), and further increased in the combined treatment group than the BMP9 treatment group (P<0.01). After inhibiting Notch signal by DAPT, the ALP staining and the expression of Heyl mRNA in the combined treatment group of BMP9+DAPT were significantly reduced compared with the separate treatment group of BMP9 (P < 0.05). Moreover, the ALP staining and the expression of Heyl mRNA in BMP9 + FSH +DAPT combined treatment group were significantly lower than that in BMP9 + FSH combined treatment group (P<0.05). Conclusion FSHβ can enhance the osteogenic differentiation of mesenchymal stem cells induced by BMP9, and Notch signaling may play an important role in it.
- Publication
Chinese Journal of Osteoporosis, 2019, Vol 25, Issue 6, p775
- ISSN
1006-7108
- Publication type
Academic Journal
- DOI
10.3969/j.issn.1006-7108.2019.06.010