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- Title
Construction and expression of spap/A eukaryotic expression plasmid of Streptococcus mutans in mammalian cells.
- Authors
QU Yun-peng; LIU Jian-guo; YANG Ping; YANG De-qin
- Abstract
PURPOSE: The study is aimed to evaluate the expression of recombinant plasmid pVAX1-spap/A of surface protein antigen A of Streptococcus mutans in mammalian cells COS-7. METHODS: The eukaryotic plasmid carrying encoding gene of spap/A of Streptococcus mutans was constructed and the plasmid introduced into COS-7 cells by lipofectamine reagent. The transient expressed protein was detected by immunochemistry technique in COS-7 cells. RESULTS: Positive expression was detected in plasma of the cells which were transfected with recombinant plasmid pVAX1-spap/A. The cells which were transfected with pVAX1 were negative. CONCLUSIONS: Spap/A can translate and express in COS-7 cells after transfected with recombinant plasmid pVAX1-spap/A. The expressed protein locates in the plasma and the protein is able to combine with anti-spap/A antibody. The expressed protein has the antigenicity and recombinant plasmid pVAXl-spap/A is a candidate vaccine. Supported by Key Research Project of Bureau of Education of Guizhou Province (Grant No.2004119).
- Publication
Shanghai Journal of Stomatology, 2009, Vol 18, Issue 1, p61
- ISSN
1006-7248
- Publication type
Academic Journal