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- Title
大豆华夏3号突变体库构建及SSR分子标记.
- Authors
黄益安; 邓小娟; 万海波; 王 朋; 方小龙; 张 杰; 杨存义
- Abstract
To provide better materials for breeding and functional gene study, mutagenesis was carried out on soybean cultivar Huaxia 3 by ethyl methane sulphonate (EMS) and seed irradiation with 60Co gamma-ray. Phenotypic traits including leaf, stem, flower, seed, pod and flowering-time variations were investigated in M4 generation. 86 mutants were identified from the population including mutants on leaf (13), stem (30), flower color (4), seed (2), pod (15), and flowering-time (22). 82 pairs of SSR markers were used to screen the genomic difference between wild type and 270 mutants. Among them, 4 mutants (M3-91, M3-500-24, EMS3-23 and EMS3-31) had more than 10 polymorphic markers. 6 mutants were different to wild type on locus Satt513.
- Publication
Chinese Journal of Oil Crop Sciences, 2016, Vol 38, Issue 2, p159
- ISSN
1007-9084
- Publication type
Academic Journal
- DOI
10.7505/j.issn.1007-9084.2016.02.004