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- Title
Expression of MLAA34-HSP70 fusion gene constructed by SOE-PCR.
- Authors
Chen Zhao; Jiaqi Zhao; Wanying Wang; Yening Fan; Chunping Ma; Donghong Zhang; Yang Lv
- Abstract
To construct the pIRES2-MLAA34-HSP70 recombinant vector and express the MLAA34-HSP70 recombinant proteins in Escherichia coli (E. coli). The MLAA34 and the HSP70 genes were extracted from U937 cells by RT-PCR, and then we amplified the fusion gene MLAA34-HSP70 by SOE-PCR and inserted it into the pIRES2-EGFP vector to construct the pIRES2-MLAA34-HSP70 recombinant vector. We amplified the fusion gene MLAA34-HSP70 successfully and identified the correctness of pIRES2-MLAA34-HSP70 recombinant vector by PCR and restriction endonuclease. Moreover, the MLAA34-HSP70 recombinant proteins expressed in E. coli were consistent with the expected molecular weight. We constructed the pIRES2-MLAA34-HSP70 recombinant vector successfully and the MLAA34-HSP70 recombinant proteins were successfully expressed by the induction of IPTG.
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2017, Vol 30, p1125
- ISSN
1011-601X
- Publication type
Academic Journal