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- Title
Development and validation of HPLC method for the determination of Candesartan in human plasma.
- Authors
Hamid, Shaista; Beg, Anwar Ejaz; Muhammad, Iyad Naeem; Hassan, Sohail; Hassan, Amir; Akram, Arfa; Alladin, Neyama
- Abstract
Candesartan (CAN), an ARB-blocker, antihypertensive, was analyzed in human plasma by a simple, accurate and precise RP-HPLC (reverse phase-High performance liquid chromatography assay method which was then validated for its accuracy, specificity and precision. The mobile phase has a constitution of acetone, diethylamine and distilled water, while Phosphoric acid was used to adjust the pH to 2.5±0.1. This mobile phase was run at 1.1ml/min and the fluorescence wavelength was set to 392 nm. A C-18 HPLC, column particle size (5 μm) Mediterranean Sea ® L x 1.D. 25cm x 4.6 mm (Supelcosil), with auto sampler injection volume of 30μl,an internal standard Valsartan was utilized for chromatographic detection. Candesartan took a retention time of 6±0.5 minutes. This method was validated by the parameters of selectivity, accuracy, precision, repeatability, reproducibility, recovery, linearity and stability. Candesartan's calibration curves were found to be linear in the range of 200ng/ml to 3.125ng/ml and the coefficient of determination (r2) was found to be 0.99. Analytical recovery obtained was above 88%. Hence, this method has been found to be useful for determining Candesartan in plasma.
- Publication
Pakistan Journal of Pharmaceutical Sciences, 2018, Vol 31, Issue 6, p2323
- ISSN
1011-601X
- Publication type
Academic Journal