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- Title
IN VITRO SHOOT REGENERATION OF Citrus nobilis Lour. FROM INTACT SEED AND COTYLEDON EXPLANTS.
- Authors
FATONAH, S.; LESTARI, W.; ISDA, M. N.; PURBA, L.
- Abstract
Siam orange (Citrus nobilis Lour.) is a fruit from Kampar Riau, Indonesia, which has a sweet flavor, is fragrant, and thin-skinned. However, plantation of siam orange is decreasing due to diseases. Preservation is required to prevent loss of siam orange by planting citrus on a large area. Plant propagation through tissue culture (in vitro culture) is an alternative approach to obtain seedlings in large quantities and potential for production of pathogen-free healthy plants. The effects of plant growth regulators of 6-benzylaminopurine (BAP) on shoot regeneration of Citrus nobilis Lour. From intact seed and cotyledon explants were studied. The explants were cultured on Murashige and Skoog medium (MS) containing 30 g/L sucrose and 8 g/L agar supplemented with treatment of plant growth regulator (PGRs) (0, 1, 2, 3, 4, and 5 mg/L of BAP). The results showed that BAP treatment increased the number of shoots from cotyledon explants, but no increase the number of shoots from intact seed explants. The difference in BAP concentration (1, 2, 3, 4, and 5 mg/L) indicates the number of shoots was not significantly different (3.6 to 4 shoots) from cotyledon explants. The growth of shoots on MS medium without supplementation with BAP produced shoots that are longer, thinner, and have lower number of leaves. The growth of shoots on MS medium containing BAP produces shorter and thicker shoots and higher number of leaves. Shoots in vitro were successfully acclimatized and established in sand, soil, and compost (1:1:1) mixture covered with transparent plastic in each polybag with a survival percentage of 93.3%.
- Publication
SABRAO Journal of Breeding & Genetics, 2018, Vol 50, Issue 2, p167
- ISSN
1029-7073
- Publication type
Academic Journal