We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
THE ABSOLUTE GENETIC INVARIABILITY OF POLYEMBRYONY SEEDLING OF DUKU 'KUMPEH' (Lansium parasiticum (Osbeck) K.C. Sahni & Bennet.), A SUPERIOR TYPE FROM JAMBI, INDONESIA.
- Authors
MURNI, P.; SYAMSUARDI; NURAINAS; MANSYAH, E.; HUDA, M.
- Abstract
Duku 'Kumpeh' is one of the superior and potential cultivar of Duku (Lansium parasiticum) that originate from a specific area in the Jambi and it becomes one of the essential sources for income of Jambi people. For sustainable utilization of this essential local germplasm of tropical fruit need the understanding of the phenomenon of polyembryony and genetic information of the plant. The study of genetic variation of multiple seedlings of duku from Kumpeh was conducted. In this study, 34 seedlings were used (14 seedlings from Kumpeh and 20 seedlings from Dusun Tuo) from two mother trees in order to collect the samples of DNA. Extracted DNA samples used a modification of CTAB method then amplified using four primers of Inter-Simple Sequence Repeat (ISSR) namely (AAC)6, (AAG)6, (AAT)10, (AG)10 and two from RAPD markers (OPA 13 and OPA 18) to asses genetic variation. Base on observed and analysis of genetic variation of samples showed that multiple seedlings of seven seeds (14 seedling individuals) from Kumpeh and ten seeds (14 seedling individuals from Dusun Tuo) have the uniform of DNA band profile and the same as those of mother trees. The existing identical of all seedlings suggested that polyembryony of duku seeds derived from the non-zygotic embryo. Thus, we concluded that the reproduction system of duku (Lansium parasiticum (Osbeck) K.C. Sahni and Bennet.) occurred asexual with apomictic reproduction.
- Publication
SABRAO Journal of Breeding & Genetics, 2019, Vol 51, Issue 2, p151
- ISSN
1029-7073
- Publication type
Academic Journal