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- Title
Antibacterial Activity of Amaranthus tricolor Extracts against Five Phytopathogenic Bacteria.
- Authors
Xia, R. Z.; Liu, S. Q.; Xie, Y.; Zhang, Y.; Wang, C.; Yin, W.; Liao, X. L.
- Abstract
Efficacy of Amaranthus tricolor extracts were investigated as new bacterial antifungal components. Antibacterial substances were successively extracted from A. tricolor by ethyl acetate. Inhibition zone method was used to study the antibacterial activity of the extract against five phytopathogenic bacteria, i.e., Xanthomonas axonopodis pv. citri, Xanthomonas oryzae pv. oryzicola, Ralstonia solanacearum, Acidovorax avenae subsp. sitrulii, and Erwinia carotovora. At the same time, the factors influencing antibacterial activity such as extraction method and extracting solvent were evaluated. Inhibition rate recorded 24.8-62.1%, with the greatest effects against X. axonopodis pv. citri and X. oryzae pv. oryzicola (62.1 and 58.7%, respectively). The extracts also inhibited X. axonopodis pv. citri in vitro. Generally, the antibacterial substances from A. tricolor were effective against all of the phytopathogens tested. Further study found that different solvents had different influences on extracts. The effective inhibition rate varied by solvent as follows: ethyl acetate > ethanol> acetone = petroleum ether; the ethyl acetate extract significantly inhibited X. axonopodis pv. citri, at a rate almost equal to the control medicament of 4 mg/ml streptomycin. Dissolved the extracts in different solvents showed that distilled water was the most stable, and the effective inhibition rate for X. axonopodis pv. citri varied as follows after 72 h: distilled water (41.2%) > ethanol-water mixture (1:5) (32.4%) > ethanol (27.2%). The results showed that the A. tricolor extracts consisted of a fat-soluble component and a water-soluble substance. Different extraction conditions also had different effects, with oscillating waves had greatest effect and ultrasonication had little impact. Obtained results revealed that extracts from leaves had higher levels of antibacterial substances than from roots and stems (leaf > root > stem).
- Publication
Egyptian Journal of Biological Pest Control, 2016, Vol 26, Issue 1, p157
- ISSN
1110-1768
- Publication type
Academic Journal