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- Title
TOWARD A STAT3 INHIBITOR IN GLIOBLASTOMA.
- Authors
Sergiu, Susman; Soritau, Olga; Buruiana, Andrei; Mitre, Andrei; Florian, Stefan Ioan
- Abstract
OBJECTIVES According to the 2016 WHO classification, glioblastoma (GBM) is classified as a grade IV, being known to be one of the most lethal brain tumours. Diffuse infiltration of tumour cells in the normal brain makes a total resection impossible, the unresected cells being responsible for recurrence. Despite the multimodal treatment used today, median survival remains very low, new therapies being needed to improve patient survival. One of these molecular findings that have kept a central place in the attention of neurooncologists is signal transducer and activator of transcription- Stat3. Elevated levels of Stat3 have been highlighted in various types of cancer, GBM included. In this study, we evaluated the in vitro effect of a Stat3 inhibitor (InhibitorX) on GBM cell lines. MATERIALS AND METHODS In this study two GBM cell lines (U252 and A 172) were subjected to various concentrations of InhibitorX in culture medium in order to determine the in vitro effect on cell growth. The concentrations of InhibitorX used were 50µM, 100µM and 200µM, alone and in combination with temozolomide (TMZ), radiotherapy and hypoxia. The inhibitory rate of cell growth was measured using MTT assay, and compared with a negative control. RESULTS The maximum inhibitory effect of InhibitorX on GBM cell lines was obtained at 200µM after 6 days of treatment. The effect on cell growth was similar to Temozolomide at 50µM concentration. Hypoxia accentuated the effect of InhibitorX on both cell lines, but we didn't note the same thing for radiotherapy and chemotherapy. CONCLUSIONS Our results showed a potent in vitro inhibitory effect on both GBM cell lines. In vivo studies and clinical approaches should consider InhibitorX as a potential therapeutic drug that can be used for GBM treatment in the future.
- Publication
Romanian Neurosurgery, 2019, Vol 33, p207
- ISSN
1220-8841
- Publication type
Academic Journal