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- Title
ÜZÜM ÇEKİRDEĞİNDEN FENOLİK BİLEŞEN EKSTRAKSİYONUNUN YÜZEY YANIT METODU İLE OPTİMİZASYONU.
- Authors
Çağdaş, Ece; Seydim, Atıf Can
- Abstract
Grape phenolics are secondary metabolites with potential beneficial effects on human health because of their antioxidant and antimicrobial activities. Thus, it is necessary to have efficient extraction techniques to achieve good recoveries of compounds. In this study, phenolic compounds of grape seeds which are byproducts of grape industry were extracted by solid-liquid extraction using water/ethanol solvent mixture. Extraction was carried out with independent variables which were solvent ratio (60-90 ml ethanol/100 ml solvent), grape seed concentration (10-20 g/100 ml solvent) and process time (32-120 min). Extraction process was optimized by using response surface methodology (RSM) for the total phenols, antioxidant and some phenolic compounds content of grape seeds. Optimal extraction conditions were identified as 60 ml ethanol/100 ml solvent (ethanol+water); 20 g grape seed/100 ml solvent; 109 min extraction time for maximum total phenols (0.254 mg/g), antioxidant activity (67.90 pmol/ml), gallic acid (0.143 mg/g), catechin (0.058 mg/g) and epicatechin (0.040 mg/g). Generally, solid concentration and extraction time were determined as the most important parameter on phenolic extraction (P<0.05).
- Publication
GIDA: The Journal of Food, 2016, Vol 41, Issue 6, p403
- ISSN
1300-3070
- Publication type
Academic Journal
- DOI
10.15237/gida.GDl6042