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- Title
Investigation of high frequency oscillations in BDNF heterozygous mouse hippocampus and entorhinal cortex.
- Authors
Abidin, İsmail; Abidin, Selcen Aydın
- Abstract
Objective: High frequency oscillations (HFO) in the central nervous system result from a combination of potential fluctuations in the neuronal membrane and changes during synaptic transmissions. Brain-induced neurotropic factor (BDNF) is involved in neuronal function and adaptive responses in the central nervous system. Its effects on neuron excitability and on synaptic transitions are well defined. The aim of our study was to investigate the effects of physiological chronic BDNF deficiency on HFO and to show whether BDNF has an effect on HFOs. Methods: This study was approved by Karadeniz Teknik University local animals ethics committee. In the study, BDNF heterozygous mice were used. 350-400 micrometer thick slices were cut from the brain tissues of 30-35 days old mice. Electrophysiological field potentials were recorded from transverse slices of the entorhinal cortex-hippocampus. Artificially synchronized discharges were induced in the slices by 4 Aminopyridine application. Recordings containing short lasting (<3 s.) discharges were filtered by applying appropriate digital filters and ripple and fast ripple fluctuations were determined. The ratio of the event that include an HFO to the all events were calculated. Ratios were statistically compared by using t-test between wild type and heterozygous mice. Results: The ratio of synchronous events including HFO in heterozygous mice was significantly less in the entorhinal cortex (p<0.05) and in the hippocampus CA1 region (p<0.05) than in the wild type mice of the same age and gender. Conclusion: Our present data shows that similar to EEG bands, chronically reduced concentrations of BDNF resulted in reduced high frequency fluctuations. As a result, our data revealed that BDNF contributes to the synchronicity of HFOs.
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue S1, pS22
- ISSN
1307-8798
- Publication type
Academic Journal