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- Title
Effect of γ-aminobutyric acid receptors inhibition on the low-frequency primed long term potentiation.
- Authors
Koşar, Bilal; Delibaş, Sümeyra; Dursun, Nurcan; Süer, Cem
- Abstract
Objective: β-aminobutyric acid (GABA) is the major inhibitory neurotransmitter that conveys its inhibitory effect through GABA-A receptors. Metaplasticity refers to activity-dependent changes in neural functions that modulate subsequent synaptic plasticity such as long-term potentiation (LTP). Much research on metaplasticity has been focused on glutamatergic excitatory synaptic system in the hippocampus, whereas the inhibitory GABAergic synaptic system has received less attention. Herein, we investigated low-frequency stimulation (LFS) - primed LTP at dentate gyrus synapses in the presence or absence of GABAA receptor antagonists. Methods: The study was carried out with the decision of Erciyes University Animal Experiments Local Ethics Committee dated 14.02.2018 and numbered 18/022. A total of 30 adult Wistar rats were enrolled in the study. After a 15-minute baseline recording, a LFS was applied to prime the LTP by application of high-frequency stimulation (HFS) protocol. Infusions of saline, picrotoxin (50 µmol) or bicuculline (20 µmol) were made for 1 hour starting from the application of HFS, using Hamilton pump. The 5-min averages of the excitatory postsynaptic potential (EPSP) slopes and population spike (PS) amplitudes 60 min after HFS were used as a measure of the metaplastic LTP reversal. Results: The input-output curves of the infusion groups were comparable to each other, as shown by the non significant interaction observed between stimulus intensity and infused drug. It was found that 1-Hz priming prevented subsequent LTP induction at dentate gyrus synapses when GABAA receptors are functional. However, a primed LTP accompanied with increased PS-LTP was observed in the presence of bicuculline (p<0.05) or picrotoxin (p<0.05) with respect to saline infusion. Conclusion: The present study provides evidence for a role of the GABAergic transmission in metaplastic control of LTP, suggesting that neurodegeneration of GABAergic hippocampal neurons could result in deficits in hippocampus-mediated learning.
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue S1, pS23
- ISSN
1307-8798
- Publication type
Academic Journal