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- Title
Effect of different types of calorie restriction on circulation miRNAs.
- Authors
Tuna, Bilge G.; Çiçekdal, Münevver Burcu; Özorhan, Ümit; Ekici, Işın D.; Tüysüz, Emre C.; Kuşkucu, Ayşegül; Bayrak, Ömer F.; Maharramov, Akif; Doğan, Soner
- Abstract
Objective: Beneficial effects of calorie restriction (CR) have been shown in variety of pathophysiological conditions such as neurological diseases, cardiovascular diseases, ageing and cancer. The aim of the present study was to test and compare the miRNA profile in whole blood due to different types of CR methods; chronic calorie restriction (CCR) and intermittent calorie restriction (ICR). In addition pathway analysis was performed. Methods: MMTV-TGF-α female transgenic mice were enrolled in different dietary groups; ad libitum (AL, have access food all the time, control, n=3), CCR (15% of CR application compared to AL, n=3) or ICR (one week 60% CR application following three weeks AL feeding in cyclic manner, n=3) groups starting at 10 until 82 weeks of mouse age. Blood samples were collected at weeks 10, 49/50 and 81/82 of mouse age and total of 3.195 miRNAs were analysed (Affymetrix microarray, ebayes ANOVA). Then, Gene ontology (GO) analysis in neurogenesis signalling pathways was performed. Animal studies were approved by Yeditepe University Animal Research Ethical Committee. Results: Compared to AL group, 11 miRNAs were differentially expressed in CCR, 39 miRNAs were differentially expressed in ICR-R and 11 miRNAs were differentially expressed in ICR-RF at week 49/50. GO analysis revealed that neural tube development, generation of neurons and positive regulation of neuron differentiation term was enriched. Compared to AL group, 6 miRNAs were differentially expressed in CCR at week 81/82. Compared to AL group, 26 miRNAs were differentially expressed in ICR-R at week 81/82. Synaptic membrane term was enriched in this comparison. Compared to AL group, 33 miRNAs were differentially expressed in ICR-RF at week 81/82. Axonal transport term was enriched. Conclusion: As results, due to different types of calorie restriction, miRNAs detected in whole blood belong to different functions were enriched such as generation of neurons, positive regulation of neuron differentiation, synaptic membrane and axonal transport term.
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue S1, pS33
- ISSN
1307-8798
- Publication type
Academic Journal