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- Title
AVALIAÇÃO MORFOMÉTRICA OVARIANA E RECUPERAÇÃO DE COMPLEXOS Cumulus oophorus DE FÊMEAS SUÍNAS PRÉ-PÚBERES POR ASPIRAÇÃO FOLICULAR E SECÇÃO DOS OVÁRIOS.
- Authors
Carvalho Neves, Andressa; Marcondes, Gabrielle; da Silva, Ingrid Caroline; Marta Loddi, Maria; Leal Karolewski, Luciana da Silva
- Abstract
The objectives of the study were to characterize the ovarian dimensions and evaluate two techniques of recovering COC's (Cumulus-oocyte complexes) from the ovaries of gilts with an average age of five months and average body weight of 80 kg. Ovaries from slaughterhouse were used (n=127), which were weighed using a digital balance and measured (length, width and height) with a caliper. Of the 127 ovaries, 38 were submitted to follicular aspiration and 20 were sectioned using a scalpel. The COC's of those 58 ovaries were classified with grades 1, 2, 3 and 4 using a stereomicroscope. The means of COC's recovered according to those techniques were compared by t test (P<0.05). The variables were correlated using the Pearson Correlation test. The average results observed were: ovarian weight 4.26 g; length 2.57 cm; width 1.99 cm and height 1.39 cm. A total of 5,871 COC's were recovered from the 58 ovaries. The percentages of each grade were as follows: 0.87%; 2.23%; 14.50% and 82.40% (grades 1, 2, 3 and 4 respectively). Of the total number of COC's collected, grade 4 was the most prevalent and significant to this experiment. Body weight and ovarian width have more influence on the quantity and quality of COC's. It is concluded that the ovarian section method provides greater recovery of COC's. When the technique of recovering COC's is used in prepubertal swine females, the recovery of grade 4 COC's prevails.
- Publication
Archives of Veterinary Science, 2019, Vol 24, Issue 3, p12
- ISSN
1517-784X
- Publication type
Academic Journal
- DOI
10.5380/avs.v24i3.58374