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- Title
Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.
- Authors
Hamady, Micah; Walker, Jeffrey J.; Harris, J. Kirk; Gold, Nicholas J.; Knight, Rob
- Abstract
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
- Publication
Nature Methods, 2008, Vol 5, Issue 3, p235
- ISSN
1548-7091
- Publication type
Academic Journal
- DOI
10.1038/nmeth.1184