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- Title
FUNCTIONAL EVALUATION OF LACTOBACILLUS PLANTARUM AND PEDIOCOCCUS ACIDILACTICITO ANTI-ALLERGY AND ANTI-INFLAMMATORY IN VITRO AND IN VIVO.
- Authors
Chuan-Ching Lan; Chang-Chi Hsieh; Yu-Shin Chen; Min-Ruei Syu; Chia-Hao Hsu; Sew-Fen Leu; Chuan-Liang Hsu; Chun-Chih Huang; Cheng-Chih Tsai
- Abstract
Three LAB strains (Pediococctis acidilactici PA320, Lactobacillus plantarum LP109 and L. platitarum LP110) were selected for testing the acid, bile salt tolerance, and adhesion capacity in this study. Furthermore, the reduced capacity of allergy immune reaction was evaluated with cells and animals. For cell tests, the three LAB strains were utilized for stimulating the macrophages RAW 264.7 and human peripheral blood mononuclear cells (PBMC). Enzyme-linked immunosorbent assay (ELISA) was applied to analyzing the cytokine generated from the simulation of LAB, such as Interferon-γ (IFN-γ), Interleukin-12 (IL-12), Interleukin-4 (IL-4), and Interleukin-10 (IL-10), to evaluate the function of LAB's anti-allergy effects. For assessing anti-allergic effects of LAB in vivo, murine model of atopic dermatitis was used. In vitro results showed the overall LAB strains enhanced Lhl cytokine secretion while the role in inhibiting Th2 cytokine secretion. The supplementation of LAB strains caused a reduction in IgE production upon ovalbumin sensitization. Significant reduction in inflammatory cytokine secretion was observed. Histological data showed that the LAB reduced ear thickness associated with skin allergy. In summary, antiallergy and anti- inflammatory effects of these three strains were clearly demonstrated in vitro and in vivo.
- Publication
International Journal of Probiotics & Prebiotics, 2016, Vol 11, Issue 2, p49
- ISSN
1555-1431
- Publication type
Academic Journal