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- Title
Protective effects of BDNF against oxidative damage and apoptosis in human spermatozoa.
- Authors
Najafi A.; Sobhani A. G. H.; Amidi F.; Sadighi Gilani M. A.; Moawad A. R.; Mikaeili S.; Fallah P.; Rezaiian Z.
- Abstract
Introduction: Oxidative Stress (OS) is a condition when there is an improper balance between the amount of pro-oxidant substances and the amount of seminal plasma antioxidant factors that can be induced by repeated cycles of centrifugation and seminal plasma antioxidants, removal during the sperm preparation in Assisted Reproductive Technology (ART) Reactive Oxygen Species (ROS) can cause decrease of motility, increase of apoptosis and impaired sperm function, and ultimately, influence on fertilization and pregnancy rate. Therefore, currently there is a great interest to the use of antioxidants to prevent ROS generation and ROSinduced apoptosis during the sperm preparation processes. Brain-derived neurotrophic factor (BDNF) is member of neurotrophin family that has anti- oxidant and anti-apoptotic effects on nervous system. Recent researches show that it also plays key role in male and female reproductive system such as spermatogenesis, nuclear and cytoplasmic maturation and embryo development. Materials and Methods: Liquefied semen samples obtained from normozoospermic men (n=25) after preparation by double wash (400x gr, 5 min) swim-up technique were divided in to two groups of control and treated group (with BDNF). Then motility was evaluated by means of CASA. Reactive oxygen species (ROS) and apoptosis were assessed by flowcytometry. Results: Addition of BDNF to the sperm media significantly increased mean total motility and progressive motility (75.54±2.63% vs. 66.81±2.48%, pO.001, and 46.18±2.2 vs. 39.631±1.97%, p<0.002, respectively). Also BDNF treatment caused decrease of H2O2 (50,01±6.08 vs. 38.31±5.250, p<0.02), necrosis (23.26±2.71 vs. 27.52±2.27, p<0.001) and apoptosis (3.26±0.4 vs. 3.76±0.55, p<0.49). Conclusion: Considering this result, BDNF treatment can be a potential tool against oxidative damage and apoptosis in human spermatozoa.
- Publication
Iranian Journal of Reproductive Medicine, 2015, p65
- ISSN
1680-6433
- Publication type
Academic Journal