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- Title
Investigation on the Effect of Captopril on the Production of IFN-γ and IL-10.
- Authors
Baheri, Mahboubeh; Dayer, Mohammad Reza; Sheykhi, Abdolkarim; elahi, Farzaneh Habib; Toyserkani, Tayebeh
- Abstract
Objective: Multiple Sclerosis, a chronic autoimmune neurodegenerative inflammatory disease, is the main reason of physical disability in young adults. Have been many efforts to find a better understanding of disease and subsequently effective drugs. Surprisingly Angiotensin converting enzyme (ACE) inhibitors have recently attracted attentions for their immunomodulatory effects, besides to their known effectiveness on hypertension. Captopril is one of the well- known ACE inhibitors which is used as an effective drug, for hypertension and congestive heart failure. Inconsistent results have been reported about the effect of captopril on cytokines. Material and Methods: Blood samples were collected from 20 patients diagnosed as MS patients, in heparinized tubes. Peripheral blood mononuclear cells (PBMCs) were extracted by the Ficoll-hypaque technique. Isolated cells were washed by PBS and then resuspended in RPMI 1640 media, containing 10% heat-inactivated FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, and cultured at 37°C in a humidified 5% CO2 incubator. The suspensions then divided in 96-well microplate at density of 2×105 cells/well. PBMCs from each patient were stimulated using 20μl of 0.5 and 2 mM concentrations of Captopril for 48h and 72h periods. Results: Based on our results lower concentration of Captopril (0.5 mM) increases the amount of IFNγ in both 48 and 72 hours' treatments, and at the same time reduces IL-10 in both time periods. Passing of time shows more severe effect in both cytokines. In higher concentrations (2 mM) Captopril reduces both IFNγ and IL-10, and time passing amplifies the reduction. Conclusion: Since IFN-γ is considered as an inflammatory cytokine, and in return Interleukin 10 is known with anti-inflammatory effects, our experiments show that using Captopril be risky for a MS patient. Our results are in consistent with some of previous data have shown that taking Captopril may increase the risk of affecting by MS. Further investigations are necessary to specify the effect of Captopril on the development of autoimmune diseases such as multiple sclerosis.
- Publication
Iranian Journal of Allergy, Asthma & Immunology, 2018, Vol 17, p198
- ISSN
1735-1502
- Publication type
Academic Journal