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- Title
Study the Effect of Ascorbic Acid on the Production of Cytokines in Multiple Sclerosis Patients.
- Authors
Baheri, Mahboubeh; Dayer, Mohammad Reza; Sheykhi, Abdolkarim; Elahi, Farzaneh Habib; Toyserkani, Tayebeh
- Abstract
Objective: Multiple Sclerosis, a chronic autoimmune neurodegenerative inflammatory disease, is the main reason of physical disability in young adults. More than 2 million people are affected worldwide and the rate of the disease is rather high, about 70 of 100000 in Tehran and Isfahan. Ascorbic Acid, known as vitamin C, is one of important required vitamins for humans, which must be received by food. One of the probable characteristics is anti-inflammatory activity. Since Cytokines as the key molecules in the process of inflammation and autoimmune diseases, can be appropriate targets to follow the process, have been used to check the effect of compounds on inflammation. Inconsistent results have been reported about the effect of captopril on cytokines. Material and Methods: Blood samples were collected from 20 patients diagnosed as MS patients, in heparinized tubes. Peripheral blood mononuclear cells (PBMCs) were extracted by the Ficoll-hypaque technique. Isolated cells were washed by PBS and then resuspended in RPMI 1640 media, containing 10% heat-inactivated FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, and cultured at 37°C in a humidified 5% CO2 incubator. The suspensions then divided in 96-well microplate at density of 2×105 cells/well. PBMCs from each patient were stimulated using 20μl of 0.5 and 2 mM concentrations of Ascorbic Acid for 48h and 72h periods. Results: Based on our results lower concentration of Captopril (0.5 mM) increases the amount of IFN-γin both 48 and 72 hours' treatments, and also increases IL-10 in both time periods. Passing of time shows weakens the effect in both cytokines. In higher concentrations (2 mM) Ascorbic Acid still increases Interferon gamma, but also starts to reduce IL-10, and time passing unbrace the changes. Conclusion: Since IFN-γ is considered as an inflammatory cytokine, and in return Interleukin 10 is known with anti-inflammatory effects, our experiments surprisingly showed strong inflammatory effect for Ascorbic Acid. Our results are in consistent with some of previous data have shown the reduction effect of vitamin C on Interleukin 10. More investigations are necessary to specify the effect of Vitamin C on the development of autoimmune diseases such as multiple sclerosis.
- Publication
Iranian Journal of Allergy, Asthma & Immunology, 2018, Vol 17, p199
- ISSN
1735-1502
- Publication type
Academic Journal