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- Title
The Effects of all-trans Retinoic Acid on Hyperglycemia-induced Activation of NF-κB and AP-1 Via RelA and c-Jun Gene Regulation.
- Authors
Mohammadpour, Zinat; Mahmoudi, Maryam
- Abstract
Objective: All-trans retinoic acid (ATRA) participates in many important biological functions including inflammatory cascades in cells. In this investigation, we studied the anti-inflammatory aspect of ATRA, by following changes in gene expression of RelA and c-Jun - the subunits of NF-κB and AP-1 transcription factors. Material and Methods: HepG2 cells were settled an overnight in high concentrations of glucose (30 mM) and insulin (1 μM) serum-free medium to mimic hyperglycemia and inflammatory circumstances. Then, cells were treated with 0. 1, 1, and 10 μM ATRA for 24h, 48 and 72h. RelA and c-Jun gene expression were assessed by using Real-time quantitative polymerase chain reaction (qRT- PCR). Results: P65 expression were increased time dependently in response of hyperglycemia status and then, decreased dose dependently after 72h exposure with 10 μM ATRA (p<0.005). In addition, c-Jun expression were fluctuate increased in hyperglycemia exposure and then, decreased after 72h treatment with10 μM ATRA (p<0.005). Whilst, incubating whit 1 μM ATRA for 48h increased c-Jun expression (p<0.005). Conclusion: Our results implied that ATRA could reduce NF-κB activity via P65 gene regulation. However, the effects on MAPK cascade via c-Jun expression is depend on ATRA doses. This study would recommend cautiously ATRA as a preventive and therapeutic agent for inflammatory conditions.
- Publication
Iranian Journal of Allergy, Asthma & Immunology, 2018, Vol 17, p207
- ISSN
1735-1502
- Publication type
Academic Journal