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- Title
The Natural Lipolytic Yeast Candida sp. RMUTSB-27 Isolated from Pineapple for Treatment of Cooking Oil Contaminated Wastewater.
- Authors
Pitchya Tangsombatvichit; Kanjana Pisapak; Paweena Suksaard
- Abstract
Some yeast species can be applied for treatment of oil-contaminated wastewater. Oil contamination is one of the most serious problems in agro-industrial countries. Fat, oil and grease (FOG) are waste products from household food preparation and industrial processing. The aims of this study are to isolate natural lipolytic yeasts from ripe pineapple and select for high lipase activity on Tween 80 agar. The result showed that yeast isolate No.27 had high lipase activity. It had oval-shaped cells with circular form, and the entire margin of the creamy colored colony had raised elevation and a rough surface. The isolate was identified as Candida sp. using the ITS specific sequencing region and named Candida sp. strain RMUTSB-27. This isolation grew well on YP medium containing 2% cooking oil waste as well as 2% glucose at 32°C. For oil-contaminated treatment, 2% palm oil waste mixed with wastewater was added Candida sp. RMUTSB-27 showed significantly reduction in pH, fat, oil and grease, chemical oxygen demand (COD), and total Kjeldahl nitrogen (TKN) for 4 days. Our results indicated that the yeast Candida sp. RMUTSB-27 is beneficial for lipid uptake and degradation of oil-contaminated wastewater and could be utilized for future green environmental treatment.
- Publication
EnvironmentAsia, 2020, Vol 13, Issue 3, p70
- ISSN
1906-1714
- Publication type
Academic Journal
- DOI
10.14456/ea.2020.43